Analysis Tools · Protein Structure Explorer

BUB1B · BUBR1 (mitotic checkpoint serine/threonine-protein kinase BUB1 beta)

UniProt O60566 · AlphaFold DB AF-O60566-F1 . A predicted model, not an experimental structure: pLDDT is model confidence, not evidence of function, and the two experimental structures the archived metrics checked do not cover residues 1002 or 1012.

Exploration
Archived result

Predicted model

Loading the public AlphaFold entry from alphafold.ebi.ac.uk…

Model loaded: Not loaded yet
Model the archived metrics used: AF-O60566-F1-model_v6 · PDB SHA-256 684e43160c431c4a30f7ab2c6b66c2a73ae1c50ef7561fa637dbb3ffe4dcd49f
Loaded file versus analysed model: Not compared yet
pLDDT legend: Very high (> 90) Confident (70–90) Low (50–70) Very low (< 50)

Residue selection highlights a residue of the full-length predicted model; the L737* button marks a truncation position for orientation only. No variant is modelled, no energy or stability is computed.

Archived structure metrics

From metrics.json at commit a434af9, reproduced byte-identically on September 10, 2026. Explore results.

Model: AF-O60566-F1-model_v6AlphaFold DB (predicted)
Sequence length: 1,050 residues
pLDDT, residue 1002 (Asn): 91.06 model confidence
SASA, residue 1002: 27.04 Å²
Relative SASA, residue 1002 (Tien 2013): 13.87 %
Backbone description, residue 1002: non-alpha/turn-or-loop; no DSSP assignmentPhi/Psi proxy, not DSSP
pLDDT, residue 1012 (Leu): 95.31 model confidence
SASA, residue 1012: 0.00086 Å²
Cα distance 1002–1012: 12.32 Å
Mean pLDDT, residues 985–1020: 89.79 model confidence
L737*: codon change and exon: TTA → TGA, exon 17, +4 base A, 73 nt to the next junctionNM_001211.6
N1002K: codon change and exon: AAT → AAG, exon 23 (last exon), +4 base GNM_001211.6

Functional context

UniProt annotations of O60566 (domains, regions, binding sites), fetched from rest.uniprot.org by the browser when the page opens. Positions in the track are sequence positions of the canonical entry.

Loading UniProt features…

Public variant evidence

Public reference allele counts and a ClinVar record, retrieved by the research repository on the dates shown and vendored at commit a434af9. No patient genotypes. Absent fields read “Not available”.

N1002K (c.3006T>G, 15-40220612-T-G)

gnomAD id: 15-40220612-T-G
Exomes: AC 1 / AN 1,461,878 · AF 6.841e-7
Genomes: not present
Dataset · retrieved: gnomad_r4 · 2026-09-08

public reference allele counts; no patient genotypes.

L737* (15-40209701-T-G)

gnomAD id: 15-40209701-T-G
Exomes: AC 115 / AN 1,461,846 · AF 7.867e-5
Genomes: AC 5 / AN 152,174 · AF 3.286e-5
Dataset · retrieved: gnomad_r4 · 2026-09-08

public reference allele counts; no patient genotypes.

ClinVar

Record: VCV004600147.1
Title: NM_001211.6(BUB1B):c.3006T>A (p.Asn1002Lys)
Retrieved: 2026-09-08

Same protein substitution; different DNA allele from project c.3006T>G. Not functional evidence.

Exact-variant literature search

Europe PMC query: (BUB1B OR BUBR1) AND (N1002K OR Asn1002Lys OR "c.3006T>G")
Hits · date: 0 · 2026-09-08

Search absence is not proof of absence. ClinVar is checked separately.

Files: gnomAD N1002 · gnomAD L737 · ClinVar · search.

Conservation panel

pairwise global BLOSUM62, gap open -10, extension -0.5; exclude sequence length <900; one representative/species Exploratory gene-name panel, not a curated phylogeny or pathogenicity calibration; most entries unreviewed. Species are not independent observations.

Species	737	1002	1012
Xenopus tropicalisF6TGK2	L729	N1002	L1011
Gallus gallusA0A8V0ZYE8	L772	N1045	L1055
Macaca mulattaH9ZCX5	L738	N1003	L1013
Canis lupus familiarisA0A8I3PXH1	L739	N1003	L1013
Bos taurusF1MWP1	L742	N1007	L1017
Mus musculusQ9Z1S0	L726	N992	L1002
Rattus norvegicusF1LMI1	L726	N992	L1002